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KMID : 0613820210310090827
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Journal of Life Science
2021 Volume.31 No. 9 p.827 ~ p.833
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Apoptosis Induction by Dendropanax morbiferus Leaves Extract in Human Breast Cancer Cells SK-BR-3 through MAPK Pathway
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Jung Gi-Hwan
Kim Sung-Hyun
Woo Joong-Seok
Yoo Eun-Seon
Lee Jae-Han
Han So-Hee
Jung Soo-Hyun
Kim Sae-Man
Kim Eun-Gee
Shin Man-Jae
Cho Ho-Min
Jung Ji-Youn
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Abstract
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Dendropanax morbiferus leaves (DPL) has been used as a medicine since ancient times in various diseases such as inflammation, diabetes, and cancer. In particular, it has been found to have anticancer effects on several types of cancer cells, but the anticancer effect on breast cancer cells SK-BR-3 has not yet been revealed. Therefore, in this study, DPL caused proliferation inhibition in breast cancer cells SK-BR-3 and the anticancer effect by inducing apoptosis was confirmed, through an in vitro experiment. In order to examine the effect of DPL on cell viability, MTT assay was performed to confirm a significant decrease in the concentration of cell viability. DAPI staining was performed to examine the effect of DPL on cellular morphological changes and increase of apoptotic bodies. To supplement this, an increase in the apoptosis rate was also confirmed through flow cytometry after staining with annexin V/PI. Western blot was performed to confirm apoptosis-related proteins. DPL increased the expression of Cleaved-PARP, Bax whereas decreased the expression of Bcl-2. Changes in the expression levels of MAPK pathway proteins p-ERK1/2, p-JNK, and p-p38 were also confirmed, and a significant increase in p-p38 was observed. These results indicated that DPL induced apoptosis, through p-p38 MAPK signal pathway in SK-BR-3 breast cancer cells.
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KEYWORD
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Apoptosis, breast cancer, Dendropanax morbifera, MAPK pathway, SK-BR-3
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